Selected Laboratory Protocols

DNA Purification for Microinjection BAC DNA Purification for Microinjection DNA Purification for Electroporation
Preparation of DNA from Tail Biopsies PCR Analysis of Tail DNA Transgene Copy Standards

Mouse Blastocyst PCR Assay

RNA Microinjection Buffer and Cas9 mRNA Reagents

In Vitro and In Vivo Assay for CRISPR/Cas9

Cel I Assay to Detect Non-Homologous Endjoining Repair

MOUSE beta globin Primers for PCR

RAT prolactin Primers for PCR

Neo Primers for PCR

lacZ Primers for PCR

Timeline for Transgenic Mouse Production/Analysis Timeline for Gene Targeted Mouse Production/Analysis lacZ Tissue Staining

Chromosome Counting


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