Mission Statement

The purpose of the University of Michigan Transgenic Animal Model Core is to provide access to transgenic animal technology to University of Michigan investigators in an efficient and effective manner.

This Includes:

  • Production of transgenic mice
  • Production of transgenic rats
  • Gene Targeting Service in ES Cells
  • Production of ES Cell-Mouse Chimeras
  • De Novo Creation of Mouse ES Cell Lines
  • Bacterial Artificial Chromosome Recombineering
  • Cryopreservation and Recovery of Cryopreserved Embryos and Sperm
  • Pathogen Free Rederivation of Mice and Rats
  • Reagents
  • Training
  • Other Services
  • Provisos
  • Full Description of Services


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    Transgenic Mouse Production
    Transgenic RAT Production

    Gene Targeting in Mouse ES Cells


    ES Cell-Mouse Chimera Production
    De Novo Mouse ES Cell Line Derivation
     Bacterial Artificial Chromosome Recombineering
    Reagents Access to cell lines and plasmids is only provided to investigators who have complete, approved material transfer agreements.

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    Training

    Embryonic Stem Cell Culture Training

    Participants will learn all techniques necessary to produce and identify embryonic stem (ES) cells that have undergone homologous recombination with a targeting vector. Research papers providing the experimental background underpinning methods used to engineer gene targeted mice will be presented and discussed daily. Laboratory training includes setting up a lab for ES cell culture, media and reagent preparation, normal ES cell culture, preparation of feeder cells, freezing and thawing of ES cells, identification of differentiated and undifferentiated ES cell morphologies, ES cell electroporation, picking ES cell clones, DNA preparation from ES cell clones in 96 well plates, and counting chromosomes in ES cell spreads.  Download a Syllabus.

    Pronuclear Microinjection Training

    Participants will learn all techniques necessary to produce transgenic mice. This includes superovulation, glass microinstrument fabrication, egg collection, identification of pseudopregnant females, surgical transfer of eggs to pseudopregnant recipients, and microinjection of DNA into the pronuclei of fertilized mouse eggs. A majority of trainees produce transgenic mice during training. Download a Syllabus.

    Rat transgenic production training is also available. Download a Syllabus.

    Blastocyst Microinjection

    Participants will learn all techniques necessary to produce embryonic stem cell-mouse chimeras. This includes superovulation, glass microinstrument fabrication, blastocyst collection, identification of pseudopregnant females, surgical transfer of blastocysts to pseudopregnant recipients, and microinjection of ES cells into the blastocoels of C57BL/6 blastocysts. A minority of trainees produce chimeric mice during training. Download a Syllabus.

    Mouse Embryo Transfer

    Participants will learn all techniques necessary to transfer mouse embryos to the reproductive tract of recipients. This includes superovulation, isolation of pathogen free fertilized eggs from infected stocks, transfer pipette fabrication, identification of pseudopregnant females, surgical transfer of eggs to pseudopregnant recipients. All trainees produce offspring from transferred eggs during training.

    Consult on design and construction of transgenes and targeting vectors; identification of transgenic founders; and breeding.


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    Other Services


    Provisos
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    Transgenic Outline| Gene Targeting Outline| List of Services | Service Description
    Genotyping Mice | Mouse Breeding Suggestions | Links | Home


    comments to: tsaunder@umich.edu