This modified buffer is designed increase yields of GC rich DNA or other "sticky" DNA from the Nucleobond column.
The buffer contains 50% formamide, 1.0 M KCl, 15% EtOH, 100 mM Tris-phosphate, pH 8.5.
Add 12.11 Tris base to 250 ml distilled water and dissolve
Add 74.55 g KCl to Tris solution
Autoclave this solution
Add 150 of absolute ethanol to cooled solution
Add 500 ml of formamide to solution
Adjust pH to 8.5 with phosphoric acid - do not overshoot pH
Adjust final volume with sterile distilled water to 1000 ml
Store at room temperature