Bacterial Artificial Chromosome
The mission of the BAC Core is to provide access
complex technology to investigators at the so that they can use their
to conduct research instead of developing tools for research.
BACs contain large segments of chromosomal
DNA (over 100,000
base pairs). The extensive DNA sequence contained in the BACs confers
significant advantages to investigators who wish to use gene expression
in their research. Shorter DNA fragments
often do not contain enough gene expression information to recapitulate
gene expression patterns (Giraldo
and Montoliu, 2001). The BAC Core uses recombineering methods
to introduce genetic modifications in BACs by homologous recombination.
BAC DNA Purification and pulsed field gel mapping request form.
BAC Recombineering request form.
Examples of research projects that take advantage
technology include establishment of transgenic mouse models of human
et al., 2000), and transgenic mouse experiments to identify
human disease genes (Probst
al., 1998, Wang
et al., 1998). The Transgenic Core has a extensive experience in
production of BAC transgenic mice (Van
Keuren et al., 2009) and transgenic rats so that the translation of
engineered BACs into genetically engineered mouse and rat models is
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